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KMID : 0644120110240040031
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Journal of Oriental Gynecology
2011 Volume.24 No. 4 p.31 ~ p.49
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Protective Effect of Polygonum Multiflorum on Cell Damage in UVB-irradiated HaCaT Keratinocytes
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Lee Seung-Ah
Yoo Dong-Youl
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Abstract
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Objectives: This study was performed to assess the protective effect of Polygonum multiflorum(PM) on UVB-irradiated HaCaT Keratinocytes damage.
Methods: The protective effects of Polygonum multiflorum(PM) were determined by UVB-irradiated HaCaT assay. We assessed protective effects of Polygonum multiflorum(PM) on LDH release and nitrite production from HaCaT. COX-2, Bcl-2, Bax, , c-jun, c-fos, NF-, iNOS, Bcl-xL gene expression were determined in HaCaT using real-time PCR method.
Results: 1. PM inhibited LDH Release in UVB-irradiated HaCaT Keratinocytes. 2. PM inhibited Nitrite Production in UVB-irradiated HaCaT Keratinocytes. 3. PM suppressed the Gene Expression of COX-2 in UVB-irradiated HaCaT Keratinocytes. 4. PM increased the Gene Expression of Bcl-2 in UVB-irradiated HaCaT Keratinocytes. 5. PM didn’t increase the Gene Expression of Bax in UVB-irradiated HaCaT Keratinocytes. 6. PM suppressed the Gene Expression of in UVB-irradiated HaCaT Keratinocytes. 7. PM suppressed the Gene Expression of c-jun in UVB-irradiated HaCaT Keratinocytes. 8. PM suppressed the Gene Expression of c-fos in UVB-irradiated HaCaT Keratinocytes. 9. PM suppressed the Gene Expression of NF- in UVB-irradiated HaCaT Keratinocytes. 10. PM suppressed the Gene Expression of i-NOS in UVB-irradiated HaCaT Keratinocytes. 11. PM didn’t increase the Gene Expression of Bcl-xL in UVB-irradiated HaCaT Keratinocytes
Conclusions: In conclusion, these results suggest that PM inhibited the cell damage in UVB-irradiated HaCaT.
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KEYWORD
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Polygonum multiflorum(PM), HaCaT Keratinocytes, LDH, nitrite, COX-2
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